TransFix/EDTA Vacuum Blood Collection Tubes (TVTs) are plastic, direct draw collection tubes used to stabilise venous blood at the point of collection and preserve whole blood specimens for immunophenotyping by flow cytometry. TVTs are prefilled with sufficient TransFix containing K3EDTA for the immediate anticoagulation and stabilisation of 3 mL or 9 mL of blood for up to 14 days.
All TVT are CE marked for IVD use in Europe.
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Features of TVTs
- Preserve the cell surface antigens of lymphocyte subsets for up to 14 days.
- Prefilled with sufficient TransFix and K3EDTA to stabilise and anticoagulate a final draw volume of 3 mL or 9 mL of blood.
- Multiple pack sizes available.
- Immediate stabilisation providing optimal sample stability.
- Ease of application: TransFix/EDTA Vacuum Blood Collection Tubes fit docking sheaths from most manufacturers.
- Sterilised by ionizing radiation.
Benefits of TVTs
- Reduced variability:
- Ensures sample integrity during transportation between testing sites.
- Reduction in pre-analysis variability – all samples preserved at the same time point.
- Reduces the impact of unexpected delays in analysis
- Convenience:
- Easy to use – directly collect blood into TransFix then just mix by inversion.
- Minimal “hands-on” time at collection sites
- Reduced cost:
- Greater efficiency in testing – allows for batching of samples prior to testing.
- Prevent the need for repeat phlebotomy due to degraded samples.
- Further tests can be performed on the same sample after the initial analysis, without subject recall.
Product Codes
| TVT-03-1 | TransFix/EDTA 3 mL Vacuum Blood Collection Tubes (1 tube) |
| TVT-03-2 | TransFix/EDTA 3 mL Vacuum Blood Collection Tubes (2 tubes) |
| TVT-03-50 | TransFix/EDTA 3 mL Vacuum Blood Collection Tubes (50 tubes) |
| TVT-09-1 | TransFix/EDTA 9 mL Vacuum Blood Collection Tubes (1 tube) |
| TVT-09-2 | TransFix/EDTA 9 mL Vacuum Blood Collection Tubes (2 tubes) |
| TVT-09-50 | TransFix/EDTA 9 mL Vacuum Blood Collection Tubes (50 tubes) |
TVT Related Articles
TransFix Stabilisation of Blood Collected on Heparin Anticoagulants
Cytomark have validated TransFix for use with EDTA anticoagulant for lymphocyte phenotyping, however there are publications documenting the successful use ...
Blood Stabilisation: The Importance in a Clinical Setting
Without stabilisation, flow cytometric analysis of blood samples must be performed within 48 hours of venepuncture, as apoptosis and proteolytic ...
Inter-laboratory Comparison of the TransFix/EDTA Vacuum Blood Collection Tube (TVT) with the 5 mL Cyto-Chex® BCT
Cytomark, a division of Caltag Medsystems, recently presented a scientific poster at the ICCS2017 conference in collaboration with the Royal Hallamshire ...
Reduce Variability in Clinical Trials by Stabilizing Samples
TransFix/EDTA Vacuum Blood Collection Tubes (TVTs) have been cleared for IVD use in the United States and Europe. Prefilled with ...
TVT Resources
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Batch 30071 (IVD)
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TransFix®: A Cellular Antigen Stabilisation Reagent
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TVT Flyer - CE IVD
Posters
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Freezing Blood Samples for Deferred Immunophenotyping
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Inter-laboratory Comparison of the TransFix/EDTA Vacuum Blood Collection Tube (TVT) with the 5 mL Cyto-Chex® BCT
Instructions
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TransFix®/EDTA Vacuum Blood Collection Tubes - Product Information Sheet (RUO)
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TransFix®/EDTA Vacuum Blood Collection Tubes - Instructions For Use (CE/IVD)
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Symbols Glossary
Further Information
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ISO 13485:2016 Certificate
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Antibodies compatible for use with TransFix
Bibliography
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WHITE PAPER: Freezing Blood Samples for Deferred Immunophenotyping
This study used the TVTs and BCTs to compare the immunophenotype of fixed peripheral whole blood samples at time zero, and after freezing at -80°C for 7 days. The structural integrity of the frozen TVTs and BCTs were also examined. Optimisation of storage conditions was further investigated to improve immunophenotyping after freezing. TVTs showed the best cell percentage differences from the control with average lymphocyte cell recovery of 77% compared to 26% for the BCTs. All three BCTs cracked during the freezing process while all of the TVTs remained intact. Storage optimisation experiments showed tubes frozen at an angle and thawed at 37°C performed best overall. These results raise the possibility that whole blood samples for immunophenotyping may be stored at -80°C for extended periods beyond 7 days. Further studies will aim to demonstrate the upper limit of time the sample will remain stable and suitable for immunophenotyping. Experiments will also be conducted to examine the suitability for use in phosphoflow assays. Material Safety Data Sheets
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TransFix Veiligheidsinformatieblad (Nederlands)
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Transfix Sikkerhetsdatablad (Norsk)
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TransFix Sikkerhedsdatablad (Dansk)
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TransFix Sicherheitsdatenblatt (Deutsche)
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TransFix Scheda di Dati di Sicurezza (Italiano)
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TransFix Säkerhetsdatablad (Svenska)
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TransFix Material Safety Data Sheet (English)
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TransFix Material Safety Data Sheet (English - US)
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TransFix Fiche de données de sécurité (Français)
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TransFix Fichas de datos de seguridad (Español)
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Transfix Ficha de dados de segurança (Portuguesa)
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TransFix Bezpecnostni list (Czech)
Certificates of Analysis/Conformity/Conformity
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Batch 472077HDY (RUO)
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Batch 472077DD9 (RUO)
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Batch 471077KLF (CE/IVD)
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Batch 461DZ3V1R (RUO)
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Batch 450KLFNPM (IVD)
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Batch 450KLFB2H (IVD)
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Batch 433077673 (CE/IVD)
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Batch 4330774FR (CE/IVD)
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Batch 432077JWT (RUO)
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Batch 432077EZR (RUO)
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Batch 432067XCN (RUO)
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Batch 432067TLT (RUO)
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Batch 421H6ZYSS (RUO)
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Batch 30071 (RUO)
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Batch 30071 (CE/IVD)
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Batch 30068 (RUO)
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Batch 30068 (IVD)
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Batch 30065 (CE/IVD)
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Batch 30062 (RUO)
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Batch 30062 (CE/IVD)
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Batch 30056 (RUO)
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Batch 30054 (RUO)
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Batch 30054 (CE/IVD)
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Batch 30053 (RUO)
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Batch 30053 (CE/IVD)
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Batch 30046 (CE/IVD)
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Batch 30037 (CE/IVD)
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Batch 30030 (RUO)
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Batch 30029 (RUO)
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Batch 30029 (CE/IVD)
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Batch 30025 (CE/IVD)
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Batch 30005 (CE/IVD)
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Batch 30005 (CE/IVD)
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Batch 30002 (RUO)
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Batch 30001 (CE/IVD)
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Batch 29991 (CE/IVD)
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Batch 29990 (CE/IVD)
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Batch 29980 (IVD)
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Batch 29957 (RUO)
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Batch 29957 (IVD)